Genetic Site (Locus)

Human Specific

16 Genetic Sites

PowerPlex® 16HS


Reference Profile















Penta D

Penta E

Mouse MarkerINCLUDED
​It is recommended that cell line authentication be performed:

  • At an early passage - in order to establish the cell line identity (especially for "in-house" created cell lines; compare to patient tissue or sample)

  • Before beginning a new series of experiments

  • Before freezing down stocks or working banks

  • When the cell line is not behaving as expected or experimental results are not reproducible

  • Every two months that the culture is actively growing, and again before publication of research using those cell lines

These steps are crucial to the success of the research project and to the acceptance of the research in the scientific community.  Please refer to the suggested references for additional details on cell line authentication.


​​​​​​​​​​Sample Types Accepted:

  • Genomic DNA (ship at ambient temperature)
  • Fresh cell pellets (ship on dry ice/ice packs)
  • Frozen cell pellets (ship on dry ice/ice packs)
  • Dried cell pellets (ship at ambient temperature)
  • Other specimens such as tissue (FFPE and fresh) and blood (inquire on shipping requirements) - additional cost may be incured

CLICK HERE for Sample Preparation Requirements

Human Cell Line Authentication Report Options:

  • Option #1 - Data Only - Excel worksheet of all analyzed STR profiles of all samples submitted in one batch plus peak data (electropherogram); great choice for routine cell line authentication testing as part of your internal quality control system; also a great choice for researchers with a tight budget.  We provide a step-by-step "how-to" document to help you determine whether your cell line is authentic or not.

  • Option #2 - DNA Profile Report of tested cell line's STR profile plus peak data (electropherogram); signed report showing STR DNA profile of submitted cell line - economical choice for when a signed report is needed. We provide a step-by-step "how-to" document to help you determine whether your cell line is authentic or not.

  • Option #3 and #4 - Comparative Analysis Report of tested cell line's STR profile compared to a reference cell line database STR profile (Option #3) or submitted reference specimen (Option #4; from donor OR earlier passage of submitted cell line) plus peak data (electropherogram) - includes percent identity and percent match calculations between the submitted cell line and a specified reference cell line or sample - all inclusive option for cell line authentication testing as this report will indicate whether the submitted cell line/sample is authenticated or not.

Not sure which option to choose?  

Check our FAQs page for help!​​

STR DNA profiling offers a fast, affordable, and effective way of human cell line authentication:
  • PowerPlex® 16 HS (Promega Corporation) -- Multiplex STR technology provides high resolution typing with 15 autosomal loci and 1 gender determination marker; test includes a mouse marker for the detection of mouse DNA
  • Highly informative and unambiguous
  • Highly sensitive - Detects as low as 300 picograms of human genomic DNA (about 50 diploid cells); can detect as low as 5% contaminating human DNA and as low as 0.5% contaminating mouse DNA
  • Rapid - Results typically available in 2 business days from receipt of samples
  • Inexpensive - ask about volume discounts
  • Generates reproducible data in a format suitable for reference cell line database comparison


What is STR DNA Profiling?

Human Cell Line Authentication Testing Services - STR DNA Profiling

*Includes a MOUSE marker*

The cell line authentication testing division of LabCorp (formerly known as Genetica Cell Line Testing - a LabCorp brand), provides reliable, fast and affordable authentication of human cancer cell lines, stem cell lines, xenografts, and more utilizing STR DNA technology; current clients include major research and cancer institutions, pharmaceutical and biotechnology companies within the United States and throughout the world.

Inadvertent cell line contamination has been a major problem for biomedical researchers with estimates that 18-36% of all cell lines are either misidentified or cross-contaminated with another cell line[1]; cell line integrity is of utmost importance since the validity of the data obtained often depends on the identity of the cell line or stem cells used. The National Institutes of Health [2] recommends validation of cell lines for grant applications and many journals such as those published by the American Association for Cancer Research (AACR)
[3] and Nature have guidelines for cell line authentication prior to publication .

The short tandem repeat DNA profiling (STR DNA profiling, STR DNA typing, STR DNA test, and also known as DNA fingerprinting) offers the greatest value for cell line identity verification (cell line authentication). The process of obtaining a DNA profile using highly polymorphic STR repeats includes the isolation of DNA from a human cell line or other research specimen, the amplification of targeted areas of that DNA, and analysis of the amplified product using a genetic analyzer. STR DNA typing generates reproducible data in a format suitable to reference cell line database or patient sample comparison. LabCorp's laboratory procedures for cell line authentication comply with the guidelines set forth by the American Type Culture Collection (ATCC) SDO Workgroup ASN-0002 Standards document [4], as well as with the requirements of other leading scientific organizations.

LabCorp provides reliable, fast, and affordable human cell line authentication services, using STR DNA typing to assist the scientific community in accurate identification of specimens used in research and drug development. Our standard human cell line authentication test involves generating STR DNA profiles of the submitted cell lines or other research specimens for 15 independent genetic sites and amelogenin [the sex identity locus].

NEW! All of our human cell line authentication report options include a single MOUSE marker for the detection of mouse DNA contamination, especially helpful for researchers who are using mouse/human xenograft models and/or human and mouse cell lines simultaneously. The test can also be used to confirm that a mouse cell line is NOT cross-contaminated with a human cell line or confirmation of the presence of mouse cells. We can detect as low as 0.5% contaminating mouse DNA. Additional human genetic STR sites may also be tested [contact us for details].

At LabCorp, we make cell line authentication easy and affordable. Simply click on the "Pricing" tab for service costs, call us or submit an information form to get started. We will then assist you in setting up your human cell line authentication test. Once we receive your samples, you will have your cell line authentication report and data typically within 2 business days!


Electropherogram (peak data)

Included FREE with every sample you submit

1-800-IDENTITY®  (433-6848)  

1-513-985-9777 International

Results You Can Trust!

LabCorp is one of the most experienced providers of DNA identity testing services worldwide.  As a leading DNA testing service provider, we are committed to providing our clients with high quality cell line authentication results delivered with unmatched service and satisfaction, product specialization and technical expertise.

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Cell Line Authentication & Research Services